Hyaluronan Synthase: New Directions for Hyaluronan Research.
نویسندگان
چکیده
منابع مشابه
HAS 2 ( hyaluronan synthase 2 )
Hyaluronan is catalyzed by three membraneembedded HA synthases, although HAS2 is the only essential gene. The isozymes are coded by 3 separate genes that, although related, are unlinked in the chromosome. Regulation occurs at trans-cription, posttranslational levels, as well as, by alternative splicing, differential sub-cellular locali-zation and epigenetic processes. Hyaluronan deposits in the...
متن کاملQuantitative continuous assay for hyaluronan synthase.
A rapid, continuous, and convenient three-enzyme coupled UV absorption assay was developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase from Pasteurella multocida (PmHAS). Activity was measured by coupling the UDP produced from the PmHAS-catalyzed transfer of UDP-GlcNAc and UDP-GlcUA to a hyaluronic acid tetrasaccharide primer with the ...
متن کاملHyaluronan synthase of chlorella virus PBCV-1.
Sequence analysis of the 330-kilobase genome of the virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases. Hyaluronan is an essential polysaccharide found in higher animals as well as in a few pathogenic bacteria. Expression of the A98R gene product in Escherichia coli indicated that the recombinant protein i...
متن کاملDeveloping fluorescent hyaluronan analogs for hyaluronan studies.
Two kinds of fluorescent hyaluronan (HA) analogs, one serving as normal imaging agent and the other used as a biosensitive contrast agent, were developed for the investigation of HA uptake and degradation. Our approach of developing HA imaging agents depends on labeling HA with varying molar percentages of a near-infrared (NIR) dye. At low labeling ratios, the hyaluronan uptake can be directly ...
متن کاملHyaluronan synthase in trabecular meshwork cells.
BACKGROUND/AIMS Hyaluronan is present in the trabecular meshwork where it is involved in the pathophysiology of aqueous outflow environment. In this study, the expression and regulation of hyaluronan synthase (HAS), which is the enzyme synthesising hyaluronan, in trabecular meshwork cells were investigated. METHODS Cultured bovine trabecular meshwork cells (BTMCs) were used. HAS expression in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 1998
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.10.23